P110 activation protein is strongly expressed in intestinal fibrosis in inflammatory bowel disease patients

Abstract Background Intestinal fibrosis is a common complication of inflammatory bowel disease (IBD) with limited diagnostic modalities to detect and determine the degree fibrosis. Fibroblast activation protein alpha (FAP) both dipeptidyl peptidase endopeptidase properties which virtually absent in healthy tissues has increased expression chronic fibrotic diseases. In this study, we investigated presence FAP intestinal caused by Crohn’s (CD) ulcerative colitis (UC). Methods For transmural surgical resection specimens were retrieved from Tytgat institute IBD biobank located at Amsterdam University Medical Center. patients undergoing an ileocecal for stricturing CD or subtotal colectomy therapy-refractory disease, right-sided hemicolectomy dysplasia non-metastasized colorectal cancer (CRC), included. All had established diagnosis (CD, UC, CRC) previous endoscopy biopsies provided informed consent prior surgery. gene levels determined using immunohistochemistry staining quantitative polymerase chain reaction (qPCR). Mass cytometry was used identify FAP–expressing cells. Results total, 59 ileal 35 colonic samples stained quantified asses (figure 1). A 1.7-fold 1.9-fold increase seen inflamed (mean 22.3, p=0.0069); stenotic 25.5 p=0.0002) ileum compared non-IBD tissue 13.1). Inflamed UC colon 26.8, p = 0.0406) showed 1.4-fold 19.2). expression, 66 42 processed qPCR analysis. 5-fold 6.4-fold found 0.011, p=0.0475) 0.014, p=0.0042) ileum, 0.278, p=0.0195) controls (means: 0.002; 0.165). Conclusion This first study reporting potential as biomarker not only CD, but also UC. Significantly Additionally, CD90+GP38+ cells have been identified most present expressing